Goat anti-ORC3L Antibody

Pep ELISA, WB, IHC

Goat anti-ORC3L Antibody
Principle Name: ORC3L
Official Symbol: ORC3L
All Name and Symbols: ORC3L; origin recognition complex, subunit 3-like (yeast); LAT; ORC3; LATHEO; IMAGE50150; origin recognition complex, subunit 3 (yeast homolog)-like; OTTHUMP00000040611; homolog of latheo, Drosophila; RP3-486L4.1; origin recognition complex, subunit 3
Accession Number(s): NP_862820.1; NP_036513.2
Peptide Sequence: QKTDHVARLTWGGC, is from C Terminus
Human Gene ID(s): 23595
Important Comments: This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently obser
Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Reference(s): Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A. latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant. Neuron 1999 May; 23(1):45-54.PMID: 10402192