Goat anti-COX1 / PTGS1 Antibody

Pep ELISA, WB

Goat anti-COX1 / PTGS1 Antibody
Principle Name: COX1 / PTGS1
Official Symbol: PTGS1
All Name and Symbols: PTGS1; COX1; COX3; PHS1; PCOX1; PGHS1; PTGHS; PGG/HS; PGHS-1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); prostaglandin G/H synthase and cyclooxygenase; RP11-542K23.6
Accession Number(s): NP_000953.2; NP_542158.1
Peptide Sequence: QDDGPAVERPSTEL, is from C Terminus
Human Gene ID(s): 5742
Important Comments: This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 60kDa and 70-75kDa bands observed in lysates of cell line U937 and approx 70-75kDa band observed in Mouse Kidney and Skeletal Muscle lysates (calculated MW of 64.5kDa according to Human NP_542158.1 and 68.7kDa Human NP_000953.2, and
Reference(s): Yokoyama C, Tanabe T. Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem Biophys Res Commun. 1989 Dec 15; 165(2):888-94.PMID: 2512924