Goat anti-SORBS1 / Ponsin (C Terminus) Antibody

Pep ELISA, IHC

Goat anti-SORBS1 / Ponsin (C Terminus) Antibody
Principle Name: SORBS1 / Ponsin (C Terminus)
Official Symbol: SORBS1
All Name and Symbols: SORBS1; PONSIN; sorbin and SH3 domain containing 1; CAP; SH3D5; SORB1; SH3P12; ponsin; sh3p12; FLJ12406; KIAA1296; DKFZP586P1422; DKFZp451C066; SH3-domain protein 5 (ponsin); FLAF2; FLJ12406; R85FL; Fas-ligand associated factor 2; c-Cbl associated protei
Accession Number(s): NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1
Peptide Sequence: GTFPGNYVKPLYL, is from C Terminus
Human Gene ID(s): 10580
Non-Human GeneID(s): 20411 (mouse);
Important Comments: This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: No signal obtained yet but low background observed in Human Heart and 293 lysates at up to 2ug/ml.
Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 2.5ug/ml.
Reference(s): Ribon V, Printen JA, Hoffman NG, Kay BK, Saltiel AR. A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes. Mol Cell Biol. 1998 Feb; 18(2):872-9.PMID: 9447983