Goat anti-FOXL2 / BPES Antibody

Pep ELISA, WB, IHC

Goat anti-FOXL2 / BPES Antibody
Principle Name: FOXL2 / BPES
Official Symbol: FOXL2
All Name and Symbols: FOXL2; BPES; forkhead box L2; PFRK; BPES1; PINTO; forkhead transcription factor FOXL2; blepharophimosis, epicanthus inversus and ptosis; Blepharophimosis, epicanthus inversus, and ptosis 1; POF3
Accession Number(s): NP_075555.1
Peptide Sequence: DSKTGALHSRLDL, is from C Terminus
Human Gene ID(s): 668
Non-Human GeneID(s): 26927 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different a
Immunohistochemistry: In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Reference(s): Crisponi L, Deiana M, Loi A, Chiappe F, Uda M, Amati P, Bisceglia L, Zelante L, Nagaraja R, Porcu S, Ristaldi MS, Marzella R, Rocchi M, Nicolino M, Lienhardt-Roussie A, Nivelon A, Verloes A, Schlessinger D, Gasparini P, Bonneau D, Cao A, Pilia G. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet. 2001 Feb; 27(2):159-66.PMID: 11175783