Goat anti-KPNA4 / IPOA3 Antibody

Pep ELISA, WB, IHC

Goat anti-KPNA4 / IPOA3 Antibody
Principle Name: KPNA4 / IPOA3
Official Symbol: KPNA4
All Name and Symbols: KPNA4; IPOA3; QIP1; SRP3; MGC12217; MGC26703; karyopherin alpha 4 (importin alpha 3); importin alpha 3; importin-alpha-Q1; karyopherin alpha 4
Accession Number(s): NP_002259.1
Peptide Sequence: NSSANVPTEGFQF, is from C Terminus
Human Gene ID(s): 3840
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 55-60kDa band observed in Human Testis lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended concentration: 0.2-1ug/ml
Immunohistochemistry: In paraffin embedded Human Testis shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration: 3ug/ml. In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocyt
Reference(s): Seki T, Tada S, Katada T, Enomoto T. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL. Biochem Biophys Res Commun. 1997 May 8; 234(1):48-53.PMID: 9168958