Goat anti-SHP2 / PTPN11 Antibody

Pep ELISA, WB, IHC

Goat anti-SHP2 / PTPN11 Antibody
Principle Name: SHP2 / PTPN11
Official Symbol: PTPN11
All Name and Symbols: PTPN11; SHP2; protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1); CFC; NS1; BPTP3; PTP2C; SHP-2; PTP-1D; SH-PTP2; SH-PTP3; Noonan syndrome 1; protein-tyrosine phosphatase 2C; protein tyrosine phosphatase, non-receptor type 11; MGC14433
Accession Number(s): NP_002825.3
Peptide Sequence: YENVGLMQQQKSFR, is from C Terminus
Human Gene ID(s): 5781
Non-Human GeneID(s): 19247 (mouse); 25622 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 70kDa band observed in Human Skeletal Muscle lysates and in lysates of cell line HEK293 (calculated MW of 68.0kDa according to NP_002825.3). In transfected HEK293 transiently expressing PTPN11 bands of approx. 70kDa and 49kDa are obs
Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong cytoplasm staining in Purkinje cells and processes. Recommended concentration, 3-6ug/ml.
Reference(s): Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T. The SH2 tyrosine phosphatase shp2 is required for mammalian limb development. Nat Genet 2000 Apr; 24(4):420-3.PMID: 10742110