Goat anti-FBL2 / FBXL2 Antibody

Pep ELISA, WB, IHC

Goat anti-FBL2 / FBXL2 Antibody
Principle Name: FBL2 / FBXL2
Official Symbol: FBXL2
All Name and Symbols: FBXL2; FBL2; F-box and leucine-rich repeat protein 2; FBL3; DKFZP564P0622; F-box protein containing leucine-rich repeats
Accession Number(s): NP_036289.2
Peptide Sequence: VFSNNDEGLINKK, is from N Terminus
Human Gene ID(s): 25827
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in Human Brain (Frontal Cortex) lysates and approx 50kDa band observed in Rat Brain lysates (calculated MW of 47.1kDa according to Human NP_036289.2, and 46.9kDa according to Rat XP_343496.4). Recommended concentr
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration, 5-10ug/ml.
Reference(s): Ilyin GP, Rialland M, Pigeon C, Guguen-Guillouzo C. cDNA cloning and expression analysis of new members of the mammalian F-box protein family. Genomics. 2000 Jul 1; 67(1):40-7.PMID: 10945468