Goat anti-MAML1 / Mastermind Antibody

Pep ELISA, WB, IHC

Goat anti-MAML1 / Mastermind Antibody
Principle Name: MAML1 / Mastermind
Official Symbol: MAML1
All Name and Symbols: MAML1; mastermind-like 1 (Drosophila); Mam1; Mam-1; KIAA0200; mastermind homolog; mastermind-like 1
Accession Number(s): NP_055572.1
Peptide Sequence: VLPTCPMAEFALPR, is from N Terminus
Human Gene ID(s): 9794
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 110kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108kDa according to NP_055572.1). Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8ug/ml.
Reference(s): Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S, Griffin JD. MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors. Nat Genet. 2000 Dec; 26(4):484-9.PMID: 11101851