Goat anti-NDRG1 Antibody

Pep ELISA, WB, IHC

Goat anti-NDRG1 Antibody
Principle Name: NDRG1
Official Symbol: NDRG1
All Name and Symbols: NDRG1; N-myc downstream regulated gene 1; CAP43, ; CMT4D; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated gene 1 protein; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; protein reg
Accession Number(s): NP_006087.2
Peptide Sequence: GNSAGPKSMEVSC, is from C Terminus
Human Gene ID(s): 10397
Non-Human GeneID(s): 17990 (mouse);
Important Comments: The variants (NP_006087.2; NP_001128714.1) encode the same protein
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). The observed molecular weight corresponds to earlier findings wit
Immunohistochemistry: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2u
Reference(s): Zhou D, Salnikow K, Costa M. Cap43, a novel gene specifically induced by Ni2+ compounds. Cancer Res. 1998 May 15; 58(10):2182-9.PMID: 9605764