Goat anti-Smooth muscle alpha-actin Antibody

Pep ELISA, WB

Goat anti-Smooth muscle alpha-actin Antibody
Principle Name: Smooth muscle alpha-actin
Official Symbol: ACTA2
All Name and Symbols: ACTA2; actin, alpha 2, smooth muscle, aorta; OTTHUMP00000020042; ACTSA; AAT6; ACTSA; alpha 2 actin; alpha-cardiac actin; growth-inhibiting gene 46; smooth muscle alpha-actin;
Accession Number(s): NP_001604.1; NP_001135417.1
Peptide Sequence: EEEDSTALVC, is from N Terminus
Human Gene ID(s): 59
Important Comments: Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 45kDa band observed in Human and Rat Duodenum lysates and approx. 40-45kDa in lysates oif cell line HeLa (calculated MW of 42kDa according to Human NP_001604.and Rat NP_112266.1). Recommended concentration: 0.01-0.3ug/ml. Primary inc
Reference(s): Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25; 265(3):1683-7. .PMID: 2295650