Goat anti-Sorbitol Dehydrogenase Antibody

Pep ELISA, WB

Goat anti-Sorbitol Dehydrogenase Antibody
Principle Name: Sorbitol Dehydrogenase
Official Symbol: SORD
All Name and Symbols: sorbitol dehydrogenase; SORD; SORD1
Accession Number(s): NP_003095.2
Peptide Sequence: KIMLKCDPSDQNP, is from C Terminus
Human Gene ID(s): 6652
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 38kDa band observed in Mouse Kidney lysates (calculated MW of 38.3kDa according to NP_003095.1). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was success
Reference(s): Karacaoglan V, Ozer I. Steady-state kinetic properties of sorbitol dehydrogenase from chicken liver. Comp Biochem Physiol B Biochem Mol Biol. 2005 Feb; 140(2):309-12. .PMID: 15649778