Goat anti-Centromere protein E Antibody

Goat anti-Centromere protein E Antibody
Principle Name: Centromere protein E
Official Symbol: CENPE
All Name and Symbols: CENPE; CENP-E; centromere protein E (312kD); Centromere autoantigen E (312kD); centromere protein E, 312kDa; KIF10; centromere protein E; kinesin family member 10
Accession Number(s): NP_001804.2
Peptide Sequence: HASSGKDVPECKTQ, is from C Terminus
Human Gene ID(s): 1062
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 316kDa according to NP_001804. Therefore we cannot recommend an optimal concentration and the produ
Reference(s): Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 1991 May; 10(5):1245-54.PMID: 2022189