Goat anti-ERK2 / MAPK1 Antibody

Pep ELISA, WB

Goat anti-ERK2 / MAPK1 Antibody
Principle Name: ERK2 / MAPK1
Official Symbol: MAPK1
All Name and Symbols: MAPK1; ERK2; ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; mitogen-activated protein kinase 1; protein tyrosine kinase ERK2; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; extracellular signal-regulated kin
Accession Number(s): NP_002736.3; NP_620407.1
Peptide Sequence: CAAGPEMVRGQVF, is from internal region
Human Gene ID(s): 5594
Non-Human GeneID(s): 26413 (mouse);
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 38kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2ug/ml.