Goat anti-AKAP9 / AKAP450 / CG-NAP Antibody

Goat anti-AKAP9 / AKAP450 / CG-NAP Antibody
Principle Name: AKAP9 / AKAP450 / CG-NAP
Official Symbol: AKAP9
All Name and Symbols: AKAP9; CG-NAP; PRKA9; YOTIAO; AKAP350; HYPERION; KIAA0803; A kinase (PRKA) anchor protein (yotiao) 9; AKAP120-like protein; kinase N-associated protein; A-kinase anchoring protein 450; A-kinase anchor protein, 350kDa; protein kinase A anchoring protein 9
Accession Number(s): NP_005742.4; NP_671714.1
Peptide Sequence: SGSTTQFHAGMR, is from C Terminus
Human Gene ID(s): 10142
Important Comments: This antibody is expected to recognise isoforms 2 and 3 (NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: This product has been successfully used by a customer, showing a band at approx 450kDa in lysates of cell line HepG2 (calculated MW of 453kDa according to NP_005742.4). Recommended concentration: 1-3ug/ml.
Reference(s): Ciampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova MN, Rabes HM, Fagin JA, Nikiforov YE. Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer. J Clin Invest. 2005 Jan; 115(1):94-101. .PMID: 15630448