Goat anti-ACHE Antibody

Goat anti-ACHE Antibody
Principle Name: ACHE
Official Symbol: ACHE
All Name and Symbols: ACHE; YT; acetylcholinesterase (YT blood group); ARACHE; N-ACHE; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase
Accession Number(s): NP_000656.1; NP_001269378.1
Peptide Sequence: QFDHYSKQDRCSDL, is from C Terminus
Human Gene ID(s): 43
Important Comments: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, FCPeptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 65-70kDa band observed in lysates of cell lines Daudi, HeLa and Jurkat, and approx. 70kDa in Rat Brain lysates (calculated MW of 67.8kDa according to Human NP_000656.1 and 68.2kDa according to Rat NP_742006.1). Recommended concentra
Reference(s): Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16; 42(36):10863-73. .PMID: 12962511