Goat anti-Lipocalin 2 / NGAL Antibody

Pep ELISA, WB, IHC

Goat anti-Lipocalin 2 / NGAL Antibody
Principle Name: Lipocalin 2 / NGAL
Official Symbol: LCN2
All Name and Symbols: LCN2; NGAL; lipocalin 2 (oncogene 24p3); HGNC:6526; uterocalin; oncogenic lipocalin 24p3; neutrophil gelatinase-associated lipocalin; lipocalin 2; MSFI; OTTHUMP00000022240; migration-stimulating factor inhibitor; neutrophil gelatinase-associated lipocalin
Accession Number(s): NP_005555.2
Peptide Sequence: AILREDKDPQKMY, is from internal region
Human Gene ID(s): 3934
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration: 2-3ug/ml.
Reference(s): Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet. 2005 Apr; 365(9466):1231-8. .PMID: 15811456