Goat anti-AGRP / Agouti-related protein Antibody

Pep ELISA, IHC

Goat anti-AGRP / Agouti-related protein Antibody
Principle Name: AGRP / Agouti-related protein
Official Symbol: AGRP
All Name and Symbols: AGRP; ART; AGRT; ASIP2; agouti related protein homolog (mouse); agouti (mouse) related protein; Agouti-related transcript, mouse, homolog of; MGC118963; agouti related protein homolog
Accession Number(s): NP_001129.1; NP_015531.1
Peptide Sequence: CRKLGTAMNPCSRT, is from C Terminus
Human Gene ID(s): 181
Important Comments: This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated s
Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1ug/ml.
Reference(s): Breen TL, Conwell IM, Wardlaw SL. Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus. Brain Res. 2005 Jan 25; 1032(1-2):141-8. .PMID: 15680952