Goat anti-Proopiomelanocortin / POMC Antibody

Pep ELISA, WB, IHC

Goat anti-Proopiomelanocortin / POMC Antibody
Principle Name: Proopiomelanocortin / POMC
Official Symbol: POMC
All Name and Symbols: POMC; proopiomelanocortin; Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating; hormone/ beta-endorphin; LPH; NPP; OTTHUMP00000119991; OTTHUMP00000200964; adrenocortic
Accession Number(s): NP_000930.1; NP_001030333.1
Peptide Sequence: NAIIKNAYKKGE, is from C Terminus
Human Gene ID(s): 5443
Important Comments: Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 29kDa band observed in lysates of cell line NIH3T3 (calculated MW of 29.4kDa according to NP_000930.1 and NP_001030333.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Immunohistochemistry: In paraffin embedded Human Anterior Pituitary Gland shows vesilulate staining in glandular cells. Recommended concentration: 2-4ug/ml.
Reference(s): Muhlhausler BS, Adam CL, Marrocco E, Findlay PA, Roberts CT, McFarlane JR, Kauter KG, McMillen IC. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005 Jan 20; [Epub ahead of print] .PMID: 15661821