Goat anti-Spleen tyrosine kinase / SYK Antibody

Pep ELISA, WB

Goat anti-Spleen tyrosine kinase / SYK Antibody
Principle Name: Spleen tyrosine kinase / SYK
Official Symbol: SYK
All Name and Symbols: SYK; spleen tyrosine kinase; HGNC:11491; DKFZp313N1010; FLJ25043; FLJ37489; OTTHUMP00000021628; OTTHUMP00000021629
Accession Number(s): NP_003168.2; NP_001128524.1
Peptide Sequence: KYLQQNRHVKDKN, is from internal region
Human Gene ID(s): 6850
Non-Human GeneID(s): 20963 (mouse); 25155 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1; NP_001128524.1 and NP_001167639.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 70kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1kDa according to NP_003168.2). Recommended concentration: 1-3ug/ml.