Goat anti-HNF4A Antibody

Pep ELISA, WB, IHC

Goat anti-HNF4A Antibody
Principle Name: HNF4A
Official Symbol: HNF4A
All Name and Symbols: HNF4A; TCF; HNF4; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; hepatocyte nuclear factor 4, alpha; HNF4-alpha; transcription factor-14; FLJ39654; HNF4alpha; MODY; MODY1; HNF4alpha10/11/12; hepatic nuclear factor 4 alpha
Accession Number(s): NP_849180.1; NP_000448.3; NP_849181.1
Peptide Sequence: RLSKTLVDMDMADY, is from N Terminus
Human Gene ID(s): 3172
Important Comments: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 55kDa and 48kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1, 52.7kDa according to Mouse NP_032287.2 and
Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2ug/ml.
Reference(s): Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR. Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish. Diabetes. 2004 Dec; 53(12):3337-41. .PMID: 15561969