Goat anti-Ghrelin preproprotein Antibody

Goat anti-Ghrelin preproprotein Antibody
Principle Name: Ghrelin preproprotein
Official Symbol: GHRL
All Name and Symbols: GHRL; MTLRP; ghrelin; ghrelin precursor; motilin-related peptide; preproghrelin; ghrelin/obestatin prepropeptide; obestatin; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; ghrelin, growth hormone secretagogue receptor liga
Accession Number(s): NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1
Peptide Sequence: QDILWEEAKEAPADK, is from C Terminus
Human Gene ID(s): 51738
Important Comments: This antibody is expected to recognise all reported isoforms.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC, ICCPeptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of this preproprotein of approx. 12.9kDa according to NP_057446.1 and of its derivatives. Therefore we cannot
Immunohistochemistry: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration, 2-5ug/ml.
Reference(s): Ghigo E, Broglio F, Arvat E, Maccario M, Papotti M, Muccioli G. Ghrelin: more than a natural GH secretagogue and/or an orexigenic factor. Clin Endocrinol (Oxf). 2005 Jan; 62(1):1-17. .PMID: 15638864