Goat anti-CFC1 / Cripto-1 Antibody

Pep ELISA, IHC

Goat anti-CFC1 / Cripto-1 Antibody
Principle Name: CFC1 / Cripto-1
Official Symbol: CFC1
All Name and Symbols: CFC1; HTX2; CRYPTIC; cripto, FRL-1, cryptic family 1; cripto-1; cryptic; cryptic gene; MGC133213; CR-1; FLJ77897
Accession Number(s): NP_115934.1
Peptide Sequence: HFTGRYCEHDQRRSEC, is from internal region
Human Gene ID(s): 55997
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the ca
Immunohistochemistry: In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration, 1-2ug/ml.
Reference(s): Bianco C, Strizzi L, Ebert A, Chang C, Rehman A, Normanno N, Guedez L, Salloum R, Ginsburg E, Sun Y, Khan N, Hirota M, Wallace-Jones B, Wechselberger C, Vonderhaar BK, Tosato G, Stetler-Stevenson WG, Sanicola M, Salomon DS. Role of human cripto-1 in tumor angiogenesis. J Natl Cancer Inst. 2005 Jan 19; 97(2):132-41. .PMID: 15657343