Goat anti-Clusterin / APOJ Antibody

Goat anti-Clusterin / APOJ Antibody
Principle Name: Clusterin / APOJ
Official Symbol: CLU
All Name and Symbols: CLU; APOJ; clusterin; complement lysis inhibitor; SP-40, 40; sulfated glycoprotein 2; testosterone-repressed prostate message 2; apolipoprotein J ; HGNC:2095; CLI; MGC24903; SGP-2; SGP2; SP-40; TRPM-2; TRPM2 ; apolipoprotein J; clusterin; complement-associ
Accession Number(s): NP_001822.2; NP_976084.1
Peptide Sequence: EKALQEYRKKHREE, is from C Terminus
Human Gene ID(s): 1191
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9
Reference(s): Xie D, Lau SH, Sham JS, Wu QL, Fang Y, Liang LZ, Che LH, Zeng YX, Guan XY. Up-regulated expression of cytoplasmic clusterin in human ovarian carcinoma. Cancer. 2005 Jan 15; 103(2):277-83. .PMID: 15578711