Goat anti-SDHB Antibody

Goat anti-SDHB Antibody
Principle Name: SDHB
Official Symbol: SDHB
All Name and Symbols: SDHB; IP; SDH; SDH1; SDHIP; succinate dehydrogenase complex, subunit B, iron sulfur (Ip); OTTHUMP00000044624; iron-sulfur subunit; FLJ92337; PGL4; SDH2; SDHIP
Accession Number(s): NP_002991.2
Peptide Sequence: ATYKEKKASV, is from C Terminus
Human Gene ID(s): 6390
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, EIA
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 31.6kDa according to NP_002991.2). Recommended concentration: 0.03-0.1ug/ml.
Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Reference(s): Selak MA, Armour SM, MacKenzie ED, Boulahbel H, Watson DG, Mansfield KD, Pan Y, Simon MC, Thompson CB, Gottlieb E. Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell. 2005 Jan; 7(1):77-85..PMID: 15652751