Description |
Goat anti-Orexin receptor 1 Antibody Principle Name: Orexin receptor 1 Official Symbol: HCRTR1 All Name and Symbols: HCRTR1; OX1R; hypocretin (orexin) receptor 1; orexin receptor; orexin receptor 1; orexin receptor-1; hypocretin receptor 1; hypocretin receptor-1 Accession Number(s): NP_001516.1 Peptide Sequence: RNWKRPSDQLGD, is from internal region Human Gene ID(s): 3061 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 8000. Western Blot: Approx 48+35kDa band observed in Human Brain lysates (calculated MW of 47.5kDa according to NP_001516.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol En Reference(s): Petersen A, Gil J, Maat-Schieman ML, Bjorkqvist M, Tanila H, Araujo IM, Smith R, Popovic N, Wierup N, Norlen P, Li JY, Roos RA, Sundler F, Mulder H, Brundin P. Orexin loss in Huntington's disease. Hum Mol Genet. 2005 Jan 1; 14(1):39-47. Epub 2004 Nov 03. .PMID: 15525658 |