Goat anti-STX1A / STX1B Antibody

Pep ELISA, WB

Goat anti-STX1A / STX1B Antibody
Principle Name: STX1A / STX1B
Official Symbol: STX1A / STX1B
All Name and Symbols: STX1A; syntaxin 1A (brain) ; HPC-1; STX1; p35-1; SYN1A; OTTHUMP00000174615; OTTHUMP00000174616; OTTHUMP00000174617; OTTHUMP00000174618; neuron-specific antigen HPC-1; syntaxin-1A; STX1B; syntaxin 1B; STX1B1; STX1B2; FP17469; OTTHUMP00000163224; syntaxin 1
Accession Number(s): NP_004594.1; NP_001159375.1; NP_443106.1
Peptide Sequence: KDRTQELRTAKD, is from N Terminus
Human Gene ID(s): 6804; 112755
Non-Human GeneID(s): 20907 (mouse); 116470 (rat);
Important Comments: Please note this product is expected to recognize the products of 2 different genes. STX1A (NP_004594.1and NP_001159375.1) and STX1B (NP_443106.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 35kDa band observed in Mouse Brain lysates (calculated MW of 33.0kDa according to NP_004594.1 and 33.2kDa according to NP_443106.1). Recommended concentration: 0.01-0.03ug/ml.
Reference(s): Kang Y, Leung YM, Manning-Fox JE, Xia F, Xie H, Sheu L, Tsushima RG, Light PE, Gaisano HY. Syntaxin-1A inhibits cardiac KATP channels by its actions on nucleotide binding folds 1 and 2 of sulfonylurea receptor 2A. J Biol Chem. 2004 Nov 5; 279(45):47125-31. Epub 2004 Aug 31. .PMID: 15339904