Goat anti-ERBB3 / HER3 Antibody

Pep ELISA, WB, IHC

Goat anti-ERBB3 / HER3 Antibody
Principle Name: ERBB3 / HER3
Official Symbol: ERBB3
All Name and Symbols: ERBB3; HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); HGNC:3431; ErbB-3; MDA-BF-1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3 ; erbB-3; LCCS2; MGC88033; receptor tyrosine-protein kinase erbB-3; v-erb-b2 avian
Accession Number(s): NP_001973.2
Peptide Sequence: HSRLFPKANAQRT, is from C Terminus
Human Gene ID(s): 2065
Non-Human GeneID(s): 13867 (mouse); 29496 (rat);
Important Comments: This antibody is expected to recognise isoform 1 (NP_001973.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 150kDa band observed in human lysates of T cell line Jurkat (calculated MW of 148kDa according to NP_001973.2). Recommended concentration: 0.1-0.3ug/ml. A minor band of unknown identity was also consistently observed at 48kDa. This
Immunohistochemistry: Paraffin embedded Human Lung. Recommend concentration: 5ug/ml.
Reference(s): van der Horst EH, Weber I, Ullrich A. Tyrosine phosphorylation of PYK2 mediates heregulin-induced glioma invasion: novel heregulin/HER3-stimulated signaling pathway in glioma. Int J Cancer. 2005 Feb 20; 113(5):689-98. .PMID: 15499613