Goat anti-Cannabinoid Receptor 1 Antibody

Goat anti-Cannabinoid Receptor 1 Antibody
Principle Name: Cannabinoid Receptor 1
Official Symbol: CNR1
All Name and Symbols: cannabinoid receptor 1 (brain); CNR1; HGNC:2159, CANN6, CB-R, CB1, CB1A, CB1K5, CNR; central cannabinoid receptor; RP1-23D17.1; CB1R; cannabinoid receptor 1 OTTHUMP00000016838; OTTHUMP00000016839
Accession Number(s): NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1
Peptide Sequence: HTSEDGKVQVTRPDQ, is from internal region
Human Gene ID(s): 1268
Non-Human GeneID(s): 12801 (mouse); 25248 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Rat
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 65kDa band observed in Rat Brain lysates (calculated MW of 52.7kDa according to Rat NP_036916.1 and 52.9kDa according to Human NP_057167.2). The observed molecular weight corresponds to earlier findings in literature with different a
Reference(s): Ledent C, Valverde O, Cossu G, Petitet F, Aubert JF, Beslot F, Bohme GA, Imperato A, Pedrazzini T, Roques BP, Vassart G, Fratta W, Parmentier M Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice. Science. 1999 Jan 15; 283(5400):401-4..PMID: 9888857