Goat anti-PTCH (C Terminus) Antibody

Goat anti-PTCH (C Terminus) Antibody
Principle Name: PTCH (C Terminus)
Official Symbol: PTCH1
All Name and Symbols: PTCH1; PTCH; patched homolog (Drosophila); HGNC:9585; BCNS; HPE7; NBCCS; PTC; PTC1; patched; patched (Drosophila) homolog; patched homolog 1 (Drosophila); RP11-435O5.3; FLJ26746; FLJ42602; PTCH11; OTTHUMP00000021709; OTTHUMP00000021710; PTCH protein +12b;
Accession Number(s): NP_000255.1; NP_001077072.1; NP_001077071.1; NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1
Peptide Sequence: ELQDVECEERPR, is from C Terminus
Human Gene ID(s): 5727
Important Comments: This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC. IF
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Immunohistochemistry: In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
Reference(s): Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Oct 3; [Epub ahead of print] .PMID: 16203740