Goat anti-PDE4D Antibody

Goat anti-PDE4D Antibody
Principle Name: PDE4D
Official Symbol: PDE4D
All Name and Symbols: PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); HGNC:8783; DPDE3; HSPDE4D; PDE4DN2; STRK1; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; dunce-like phosphodiesterase E3; phosphodie
Accession Number(s): NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1
Peptide Sequence: QPEACVIDDRSPDT, is from C Terminus
Human Gene ID(s): 5144
Important Comments: This antiobody is expected to recognize all reported isoforms.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf, IHC, EIA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. Calculated
Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5ug/ml.
Reference(s): Houslay MD. The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target. Mol Pharmacol. 2005 Sep; 68(3):563-7.PMID: 15958394