Goat anti-PIM2 (Internal) Antibody

Goat anti-PIM2 (Internal) Antibody
Principle Name: PIM2 (Internal)
Official Symbol: PIM2
All Name and Symbols: PIM2; pim-2 oncogene; HGNC:8987; proto-oncogene Pim-2 (serine threonine kinase); serine/threonine protein kinase pim-2
Accession Number(s): NP_006866.2
Peptide Sequence: PPGGKDREAFEAE, is from internal region
Human Gene ID(s): 11040
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended con
Reference(s): Hammerman PS, Fox CJ, Cinalli RM, Xu A, Wagner JD, Lindsten T, Thompson CB. Lymphocyte transformation by Pim-2 is dependent on nuclear factor-kappaB activation. Cancer Res. 2004 Nov 15; 64(22):8341-8. .PMID: 15548703