Goat anti-CBX5 / HP1-Alpha Antibody

Goat anti-CBX5 / HP1-Alpha Antibody
Principle Name: CBX5 / HP1-Alpha
Official Symbol: CBX5
All Name and Symbols: CBX5; HP1-ALPHA; chromobox homolog 5 (HP1 alpha homolog, Drosophila); HGNC:1555; HP1; HP1Hs-alpha ; chromobox homolog 5 (Drosophila HP1 alpha); Heterochromatin protein-1; HP1A; antigen p25; heterochromatin protein 1 homolog alpha; heterochromatin protein
Accession Number(s): NP_036249.1; NP_001120793.1; NP_001120794.1
Peptide Sequence: NKRKSNFSNSADDIK, is from internal region
Human Gene ID(s): 23468
Non-Human GeneID(s):
Important Comments: Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, FC
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room tem
Immunohistochemistry: In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.
Reference(s): Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E. Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. J Virol. 2003 May; 77(9):5415-27. .PMID: 12692243