Goat anti-14-3-3 sigma / Stratifin Antibody

Pep ELISA, WB, IHC

Goat anti-14-3-3 sigma / Stratifin Antibody
Principle Name: 14-3-3 sigma / Stratifin
Official Symbol: SFN
All Name and Symbols: SFN; 14-3-3sigma; stratifin; HGNC:10773; YWHAS
Accession Number(s): NP_006133.1
Peptide Sequence: DLHTLSEDSYKDST, is from internal region
Human Gene ID(s): 2810
Non-Human GeneID(s): 55948 (mouse); 313017 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.03-0.1ug/ml.
Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml.
Reference(s): Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr; 15(4):237-46. Review.PMID: 15857578