Goat anti-Stearoyl-CoA desaturase Antibody

Goat anti-Stearoyl-CoA desaturase Antibody
Principle Name: Stearoyl-CoA desaturase
Official Symbol: SCD
All Name and Symbols: SCD; SCD1; HGNC:10571; FADS5; PRO0998 ; acyl-CoA desaturase; delta-9-desaturase; fatty acid desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase
Accession Number(s): NP_005054.3
Peptide Sequence: RIKRTGDGNYKSG, is from C Terminus
Human Gene ID(s): 6319
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC, FC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10ug/ml.
Reference(s): Zhang S, Yang Y, Shi Y. Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J. 2004 Dec 20; [Epub ahead of print] .PMID: 15610069