Goat anti-PDK1 Antibody

Pep ELISA, WB

Goat anti-PDK1 Antibody
Principle Name: PDK1
Official Symbol: PDK1
All Name and Symbols: PDK1; pyruvate dehydrogenase kinase, isoenzyme 1; HGNC:8809; mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; pyru
Accession Number(s): NP_002601.1
Peptide Sequence: DFKDKSAEDAK, is from internal region
Human Gene ID(s): 5163
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Approx 65-70kDa band observed in Rat Heart lysates (calculated MW of 49.2kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended conce
Reference(s): Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18; 307(5712):1098-101. .PMID: 15718470