Goat anti-DCP1A Antibody

Goat anti-DCP1A Antibody
Principle Name: DCP1A
Official Symbol: DCP1A
All Name and Symbols: DCP1A; decapping enzyme ; HSA275986; Nbla00360; SMAD4IP1; SMIF; Smad4-interacting transcriptional co-activator; decapping enzyme hDcp1a; putative protein product of Nbla00360; transcription factor SMIF
Accession Number(s): NP_060873.3
Peptide Sequence: QVLTKNKDNHN, is from C Terminus
Human Gene ID(s): 55802
Non-Human GeneID(s): 75901 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 75kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3ug/ml. An additional band of 48kDa was consistently observed, however this band was not blocked
Reference(s): Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol. 2004 Apr; 165(1):31-40. Epub 2004 Apr 5. .PMID: 15067023