Goat anti-ROBO3 (internal) Antibody

Goat anti-ROBO3 (internal) Antibody
Principle Name: ROBO3 (internal)
Official Symbol: ROBO3
All Name and Symbols: ROBO3; roundabout, axon guidance receptor, homolog 3 (Drosophila) ; HGNC:13433; FLJ21044; HGPPS; RBIG1; RIG1 ; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3
Accession Number(s): NP_071765.2
Peptide Sequence: RKDGARLKEEEGR, is from internal region
Human Gene ID(s): 64221
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, FC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s
Reference(s): Jen JC, Chan WM, Bosley TM, Wan J, Carr JR, Rub U, Shattuck D, Salamon G, Kudo LC, Ou J, Lin DD, Salih MA, Kansu T, Al Dhalaan H, Al Zayed Z, MacDonald DB, Stigsby B, Plaitakis A, Dretakis EK, Gottlob I, Pieh C, Traboulsi EI, Wang Q, Wang L, Andrews C, Ya Mutations in a human ROBO gene disrupt hindbrain axon pathway crossing and morphogenesis. Science. 2004 Jun 4; 304(5676):1509-13. Epub 2004 Apr 22. .PMID: 15105459