Goat anti-Ku80 / XRCC5 Antibody

Pep ELISA, WB, IHC

Goat anti-Ku80 / XRCC5 Antibody
Principle Name: Ku80 / XRCC5
Official Symbol: XRCC5
All Name and Symbols: XRCC5; KU80; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa) ; HGNC:12833; KARP-1; KARP1; Ku86; NFIV ; ATP-dependent DNA helicase II; DNA repair protein XRCC5; Ku86 autoantigen r
Accession Number(s): NP_066964.1
Peptide Sequence: SLAKKDEKTDTLED, is from internal region
Human Gene ID(s): 7520
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of 75kDa was observed consistent with products from other sources.
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Reference(s): Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005 Mar 18; 280(11):10827-33. Epub 2005 Jan 7. .PMID: 15640154