Goat anti-UCP1 Antibody

Goat anti-UCP1 Antibody
Principle Name: UCP1
Official Symbol: UCP1
All Name and Symbols: UCP1; uncoupling protein 1 (mitochondrial, proton carrier); HGNC:12517; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; uncoupling protein 1
Accession Number(s): NP_068605.1
Peptide Sequence: EQLKRELSKSRQ, is from C Terminus
Human Gene ID(s): 7350
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 35kDa band observed in Human Adipose lysates (calculated MW of 33.0kDa according to NP_068605.1). Recommended concentration: 0.1-1 ug/ml. Primary incubation was 1 hour.
Reference(s): Ishigaki Y, Katagiri H, Yamada T, Ogihara T, Imai J, Uno K, Hasegawa Y, Gao J, Ishihara H, Shimosegawa T, Sakoda H, Asano T, Oka Y. Dissipating excess energy stored in the liver is a potential treatment strategy for diabetes associated with obesity. Diabetes. 2005 Feb; 54(2):322-32. .PMID: 15677488