Goat anti-Amphiphysin / AMPH Antibody

Pep ELISA, WB, IHC

Goat anti-Amphiphysin / AMPH Antibody
Principle Name: Amphiphysin / AMPH
Official Symbol: AMPH
All Name and Symbols: AMPH; amphiphysin; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) ; HGNC:471; AMPH1
Accession Number(s): NP_001626.1; NP_647477.1
Peptide Sequence: KRKDESRISKAE, is from internal region
Human Gene ID(s): 273
Non-Human GeneID(s): 218038 (mouse);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997
Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4ug/ml.
Reference(s): Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet. 2005 Apr; 365(9468):1406-11. .PMID: 15836889