Goat anti-NONO / p54NRB Antibody

Goat anti-NONO / p54NRB Antibody
Principle Name: NONO / p54NRB
Official Symbol: NONO
All Name and Symbols: NONO; P54NRB ; non-POU domain containing, octamer-binding ; HGNC:7871; NMT55; NRB54; P54; Nuclear RNA-binding protein, 54-kD; non-POU-domain-containing, octamer-binding; non-Pou domain-containing octamer (ATGCAAAT) binding protein
Accession Number(s): NP_031389.3
Peptide Sequence: NRAAPGAEFAPNK, is from C Terminus
Human Gene ID(s): 4841
Non-Human GeneID(s): 53610 (mouse); 317259 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2). In transfected HEK293 transiently expressing Human NONO a band of approx. 55kDa is obser
Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in select splenocytes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Reference(s): Brown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science. 2005 Apr 29; 308(5722):693-6. .PMID: 15860628