Goat anti-Leptin Receptor Antibody

Pep ELISA, WB, IHC

Goat anti-Leptin Receptor Antibody
Principle Name: Leptin Receptor
Official Symbol: LEPR
All Name and Symbols: LEPR ; leptin receptor ; CD295; OBR ; OB receptor; LeprB
Accession Number(s): NP_002294.2; NP_001003679.1; NP_001003680.1
Peptide Sequence: TQDDIEKHQSDAG, is from internal region
Human Gene ID(s): 3953
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 150kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). The observed molecular weight corresponds to earlier
Immunohistochemistry: Paraffin embedded Human Liver and Heart. Recommended concentration: 4ug/ml.
Reference(s): Snoussi K, Strosberg AD, Bouaouina N, Ben Ahmed S, Helal AN, Chouchane L. Leptin and leptin receptor polymorphisms are associated with increased risk and poor prognosis of breast carcinoma. BMC Cancer. 2006 Feb 20; 6:38. .PMID: 16504019