Goat anti-Chat (mouse) Antibody

Pep ELISA, WB, IHC

Goat anti-Chat (mouse) Antibody
Principle Name: Chat (mouse)
Official Symbol: Chat
All Name and Symbols: Chat; choline acetyltransferase ; B230380D24Rik
Accession Number(s): NP_034021.1
Peptide Sequence: KERARGPTKPSNLD, is from C Terminus
Non-Human GeneID(s): 12647 (mouse); 432359 (rat);
Important Comments: This antibody is NOT expected to cross-react to Human CHAT.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 75-80kDa band observed in Mouse Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5ug/ml.
Immunohistochemistry: Frozen sections of Mouse Basal Forebrain shows staining of neural cell bodies. Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr; 97(2):515-26. Epub 2006 Mar 15. .PMID: 16539660 [PubMed - in process