Goat anti-NET1 / ARHGEF8 (Internal) Antibody

Pep ELISA, IHC

Goat anti-NET1 / ARHGEF8 (Internal) Antibody
Principle Name: NET1 / ARHGEF8 (Internal)
Official Symbol: NET1
All Name and Symbols: NET1; ARHGEF8; neuroepithelial cell transforming gene 1; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding prot
Accession Number(s): NP_005854.2; NP_001040625.1
Peptide Sequence: DKLEYLDEKQRDPR, is from internal region
Human Gene ID(s): 10276
Non-Human GeneID(s): 56349 (mouse); 307098 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.
Reference(s): Qin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA. Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. J Biol Chem. 2005 Mar 4; 280(9):7603-13. Epub 2004 Dec 16. .PMID: 15611121