Goat anti-Ezrin / villin 2 Antibody

Pep ELISA, WB, IHC

Goat anti-Ezrin / villin 2 Antibody
Principle Name: Ezrin / villin 2
Official Symbol: VIL2
All Name and Symbols: villin 2 (ezrin) ; ezrin; VIL2; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584 ; cytovillin; villin 2
Accession Number(s): NP_003370.2
Peptide Sequence: QDYEEKTKKAER, is from internal region
Human Gene ID(s): 7430
Non-Human GeneID(s): 22350 (mouse); 54319 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 80kDa band observed in Human Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Ph
Immunohistochemistry: In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Reference(s): Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17; 281(7):4354-63. Epub 2005 Dec 13. .PMID: 16352594