Goat anti-Catalase / CAT Antibody

Pep ELISA, WB, IHC

Goat anti-Catalase / CAT Antibody
Principle Name: Catalase / CAT
Official Symbol: CAT
All Name and Symbols: CAT; catalase ; HGNC:1516; MGC138422; MGC138424
Accession Number(s): NP_001743.1
Peptide Sequence: DKYNAEKPKNAIHT, is from internal region
Human Gene ID(s): 847
Non-Human GeneID(s): 12359 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 60kDa band observed in Human Kidney lysates (calculated MW of 59.8kDa according to NP_001743.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 2ug/ml.
Reference(s): Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS. Extension of murine life span by overexpression of catalase targeted to mitochondria. Science. 2005 Jun 24; 308(5730):1909-11. Epub 2005 May 5. .PMID: 15879174