Goat anti-P27KIP1 / CDKN1B (C Terminus) Antibody

Goat anti-P27KIP1 / CDKN1B (C Terminus) Antibody
Principle Name: P27KIP1 / CDKN1B (C Terminus)
Official Symbol: CDKN1B
All Name and Symbols: CDKN1B; P27KIP1; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; CDKN4; cyclin-dependent kinase inhibitor 1B; p27(KIP1); KIP1; p27; MEN1B; MEN4; P27KIP1
Accession Number(s): NP_004055.1
Peptide Sequence: EQTPKKPGLRRRQT, is from C Terminus
Human Gene ID(s): 1027
Non-Human GeneID(s): 12576 (mouse); 83571 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IP
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 26-27kDa band observed in Human Breast and Prostate lysates (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature (Xia et al, Proc Natl Acad Sci U S A. 2005 S
Reference(s): Toyoshima H, Hunter T p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell. 1994 Jul 15; 78(1):67-74..PMID: 8033213