| Description |
Goat anti-PTCH (Internal) Antibody
Principle Name: PTCH (Internal)
Official Symbol: PTCH
All Name and Symbols: PTCH; patched homolog (Drosophila); RP11-435O5.3; BCNS; FLJ42602; HPE7; NBCCS; PTC; PTC1; PTCH1 ; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; patched; patched (Drosophila) homolog
Accession Number(s): NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1
Peptide Sequence: HPESRHHPPSNPRQQ, is from internal region
Human Gene ID(s): 5727
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1). Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Reference(s): Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T. Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays. Hum Mol Genet. 2005 Nov 15; 14(22):3379-88. Epub 2005 Oct 3. .PMID: 16203740
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