Description |
Goat anti-CIAS1 / Cryopyrin (Internal) Antibody Principle Name: CIAS1 / Cryopyrin (Internal) Official Symbol: NLRP3 All Name and Symbols: CIAS1; cold autoinflammatory syndrome 1; AGTAVPRL; AII/AVP; C1orf7; FCAS; FCU; MWS; NALP3; PYPAF1 ; AII/AVP receptor-like; Muckle-Wells syndrome; NACHT, LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; angiotensin/vasopressin recep Accession Number(s): NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1 Peptide Sequence: DLYEKAKRDEPK, is from internal region Human Gene ID(s): 114548 Non-Human GeneID(s): Important Comments: This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 64000. Western Blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat Immunohistochemistry: In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4ug/ml. Reference(s): Kanneganti TD, Ozoren N, Body-Malapel M, Amer A, Park JH, Franchi L, Whitfield J, Barchet W, Colonna M, Vandenabeele P, Bertin J, Coyle A, Grant EP, Akira S, Nunez G. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3. Nature. 2006 Mar 9; 440(7081):233-6. Epub 2006 Jan 11. .PMID: 16407888 |