Goat anti-APBB1 / FE65 Antibody

Pep ELISA, WB

Goat anti-APBB1 / FE65 Antibody
Principle Name: APBB1 / FE65
Official Symbol: APBB1
All Name and Symbols: APBB1; FE65; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); MGC:9072; RIR ; adaptor protein FE65a2; amyloid beta A4 precursor protein-binding, family B, member 1; stat-like protein
Accession Number(s): NP_001155.1; NP_663722.1
Peptide Sequence: GSLKPKRLGAHTP, is from C Terminus
Human Gene ID(s): 322
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 100kDa band observed in lysates of NIH/3T3 and HeLa (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different
Reference(s): Hoe HS, Magill LA, Guenette S, Fu Z, Vicini S, Rebeck GW. FE65 Interaction with the apoE receptor ApoEr2. J Biol Chem. 2006 Apr 25; [Epub ahead of print] .PMID: 16638748